Personalised bio-profiling of respiratory disease is lacking significantly despite a quick uptake on biotherapeutics. Bronchoalveolar lavage (BAL) cells provide some of the most valuable and targetable immune profile information for patients with respiratory diseases, yet, are rarely multiplexed. We, therefore developed a method to quantify an array of proteins that also considers the four primary cell types of BAL using NanoStrings GeoMx. Overall, 21 Asthma-matched BAL cytospins (Healthy: n=8, Mild-Asthma: n=7, Severe-Asthma: n=6) and 20 COPD-matched BAL cytospins (Healthy: n=4, Smokers: n=8, COPD: n=8) underwent morphology staining to identify the macrophages, neutrophils and eosinophils. Lymphocytes were identified as ‘the rest’ of the SYTO-13 stained nuclei. Multiple protein panels assessing the immune profile, immune cell types, cell death markers and MAPK pathway were combined to quantify up to 50 proteins per cell type per patient. The high resolution quantification identified several novel markers that distinguish asthma from healthy including low levels of S6 and high levels of BIM on asthma macrophages and low levels of fibronectin on asthma neutrophils. Deeper analysis found that BAL macrophage profiles were best at distinguishing mild asthma from healthy (>p=0.01) or severe asthma (>p=0.0001) while distinct neutrophil profiles were best at distinguishing severe asthma from healthy (p=0.01) and mild asthma (p=0.0001). Interestingly, all COPD BAL samples contained multiple Siglec8+ cell types like seen in severe asthma but not in mild asthma. To our knowledge, this is the first high-plex protein profiling of each BAL cell type comparing patients with asthma or COPD to healthy individuals. Current analysis strongly highlights the importance of understanding the subtypes of cells present in the airways, not just the presence of absence of a given cell type. Lastly, we believe this method could greatly enhance the analysis of any highly valuable sample preserved as mixed cell cytospins.